Posted: May 22nd, 2023

FRET Detection or DNA Molecules Essay

The article “Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores” is a 2010 publication in the Review of Scientific Instruments journal volume 81 from page 014301-7. The article is authored by Soni Gautam V., Singer Alon, Yu Zhiliang, Sun Yingjie, McNally Ben and Mellera Amit. Soni and colleagues discuss a new way of integrating “total internal reflection microscopy and electrical detection of biomolecules using nanopores” (p. 1) with both techniques being recognized as modalities of measuring single-molecules. This integration enables visualization of DNA and DNA-protein complexes while they undergo translocation via a nanopore. A good temporal resolution of about 1000 frames is produced while an excellent signal to background is enabled.

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