Posted: May 22nd, 2023
E.Z.N.A commercial kit is one of the greatest innovations in molecular biology. It helps in the extraction and purification of nucleic, deoxyribonucleic, and ribonucleic acids. The extraction is of great importance to researchers who aim at utilising deoxyribonucleic acid (DNA) from a variety of sources. The kit can be used in extracting the two molecules from their origins. The sources include plant materials, animal tissue, and soil. In this paper, the researcher sought to investigate the effectiveness of using the kit for the purposes of optimising the extraction of DNA from marine soils. To this end, two variables were analysed. The first was the quality of the acid yielded, while the second was the quantity that was extracted. Disruption of the cells was needed to obtain the acid. Bead beating was the process used to achieve this. Gel electrophoresis was also used to check for DNA fragmentation following this disturbance process. The research proved that the kit improved the quality of the DNA produced. The quantity of the material within a given volume of soil sample also increased. The kit was found to effectively extract DNA from an 8 gram sample of soil. The effectiveness was not achieved when a sample of 4 grams was used.
Posted: May 22nd, 2023
From the discussion above, it can be noted that it is not entirely correct to say that touching an electric wire is dangerous as this depends on the type of wire touched as well as the kind of circuit in place. In essence, the kind of wire touched is essential. For instance, one can touch the grounded neutral wire since it has a common potential difference. However, it is not safe to touch a live or hot wire because of its high potential difference, which can lead to discharge of electrons through the body. However, given serious nature of electric shock, it is necessary to take precaution. Precaution informs that it is dangerous to touch electric circuit unless there is reasonable evidence to show that the circuit is open. An open circuit does not discharge electrons since there is no potential difference across the circuit. However, when the circuit is completed (usually by connecting the switch) the circuit becomes dangerous to human beings.
Before touching an electric circuit, one should ensure that the circuit is open, additionally, one should confirm if the circuit is grounded. A grounded circuit can be touched at the common point where there is no potential difference. However, ungrounded circuits are quite dangerous especially in alternating circuits, which may be grounded far away from the point of touch thereby causing potential difference. It should also be noted that birds do not experience shock when they touch on a complete circuit because they have only one point of contact. In essence, there exists no potential difference since birds touch on a common point.
Touching wires in a complete electric circuit is dangerous. However, touching wires in an open electric circuit is safe. Therefore, it is essential that individuals confirm that the circuit is open before touching. However, if one has to touch the wires in a complete circuit, then that individual should do so with an appropriate insulation. Wires in an electric circuit are dangerous to humans because electric current can be discharged through them. Electric current has the capacity to burn vital body organs. Additionally, it can overload nerve cells thereby causing paralysis. In short, one should consider all the safety measures before touching an electric circuit
Posted: May 22nd, 2023
E.Z.N.A commercial kit is one of the greatest innovations in molecular biology. It helps in the extraction and purification of nucleic, deoxyribonucleic, and ribonucleic acids. The extraction is of great importance to researchers who aim at utilising deoxyribonucleic acid (DNA) from a variety of sources. The kit can be used in extracting the two molecules from their origins. The sources include plant materials, animal tissue, and soil. In this paper, the researcher sought to investigate the effectiveness of using the kit for the purposes of optimising the extraction of DNA from marine soils. To this end, two variables were analysed. The first was the quality of the acid yielded, while the second was the quantity that was extracted. Disruption of the cells was needed to obtain the acid. Bead beating was the process used to achieve this. Gel electrophoresis was also used to check for DNA fragmentation following this disturbance process. The research proved that the kit improved the quality of the DNA produced. The quantity of the material within a given volume of soil sample also increased. The kit was found to effectively extract DNA from an 8 gram sample of soil. The effectiveness was not achieved when a sample of 4 grams was used.
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