Posted: May 22nd, 2023

Real time PCR is one of the most powerful

Real time PCR is one of the most powerful and reliable techniques of gene analysis. It is commonly applied in the quantification of mRNA either from endogenous genes or transfected genes of either stable or transient transfection (Fricker et al. 772). RT-qPCR is arguably the most sensitive technique in the analysis of mRNA yet. When using “real-time PCR to study gene expression, scientists often investigate the decreases and increases in the intensity by which certain genes are expressed” (Livak and Schmittgen 404). This is done by measuring the abundance or deficiency of gene-specific transcripts (Fricker et al. 772). This forms the basis of action for this technique of gene analysis. In this experiment, sample tissues were collected and maintained at favorable conditions to ensure the viability of gens. Genes were extracted from the endoplasmic reticulum, which is located intracellular, of the respective tissue cells.