Posted: May 22nd, 2023

The contents were incubated for 10 minutes at temperatures

100ul DS buffer was added to the lysed materials and mixed thoroughly using a vortex. The contents were incubated for 10 minutes at temperatures of 7⁰C. Later on, the contents were centrifuged at 3000 rotations per minute for three minutes. 800ul of the supernatant was placed in a 2ml microfuge tube, where 270ul P2 buffer was added. The contents were mixed thoroughly. The contents were incubated for five minutes. After this, they were then centrifuged at 14000 rotations per minute at 4⁰C for five minutes. The aim was to remove some of the proteins and contaminants. 70% isopropanol was then added. Mixing was then done by inverting the tube for 25 minutes. Isopropanol helped precipitate the DNA as it is not soluble in it. The DNA formed pellets by aggregating together after application of isopropanol. The contents were then centrifuged at 14000 revolutions per minute at 4⁰C for 10 minutes. The supernatant was drawn from the tube slowly to avoid upsetting the DNA pellet.